Multiple Choice Questions (MCQs)
Two sets of primers instead of one pair are uses in ________.
Suicide PCR is used in ________.
Two pair of enzymes are used in ________ PCR.
The extension rate of Taq Polymerase enzyme is ________.
In ________ is a polymerase chain reaction that actually takes place inside the cell on a slide
Enzyme that creates phosphodiester bond between 3' end of one DNA segment and the 5' another is known as ________.
Real Time PCR is used to measure the quantity of a ________.
In ________ PCR there is very low probability of non-specific amplification of DNA.
Allele-specific PCR used for identifying of ________.
Melting of DNA template results in yielding ________ of DNA.
Agarose extracted from sea weeds finds use in ________.
The limitations of PCR are ________.
________ is used as a template to produce DNA in Reverse Transcripatase PCR.
Primers used for the process of polymerase chain reaction are ________.
AFLP is a highly sensitive PCR-based method for detecting ________ in DNA.
________ is used for the screening of bacterial of yeast clones for correct ligation or plasmid products.
To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.
If the primer sequence is TCG, what section of DNA will it anneal?
________ PCR uses primers whose 3' ends encompass the SNP.
________ tools have also allowed to perform prenatal genetic diagnosis.
Parental genetic diagnosis is not important for identification of ________.
Binding of primers to the single stranded DNA is called ________.
________ PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.
Non genomic material such as RNA can be detected by ________.
Denaturation is the process of ________.
________ tools have also allowed to perform penatal genetic diagnosis.
Molecular Detection methods can facilitate in detection of ________ in plants, animals and environment.
A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________.
________ enzymes have symmetrical recognition sequences but are of little value.
A recognition sequence usually ranges from ________ bases.
At what temperature does denaturation of DNA double helix takes place?
A technique for making millions of copies of a specific region of DNA.
________ PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.
The instrument used to run the PCR is known as ________.
Inverse PCR is used for the identification and amplification of ________.
Degenerate Primers are used for the amplication of ________.
What happens during the Anneal step of PCR?
At what temperature does annealing of DNA and primer take place?
________ enzymes have symmetrical recognition sequencesbut are of little value.
Genomic fingerprinting is a ________ medicine technique used to identify individuals by characteristics of their DNA.
Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ________ nucleotides.
Assembly PCR is used to assemble two or more places of DNA into ________.
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
Methylation-specific PCR is used to identify patterns of DNA methylation at ________.
For making cDNA ________ is used as a template.
________ refers to the study of ancient human, animal and plant remains.
Sequences of nucleotides which have a twofold axis of rotational symmetry is known as ________.
The process of reverse transcription reverse transcribes ________.
Which one of the following is the source of pfu Polymerase?
Which of the following statements are true regarding PCR?
What is the function of a primer?
Inverse PCR supports the amplification and identification of ________.
Why are vent ploymerase and Pfu more efficient than the Taq polymerase?
What happens in the Denature step of PCR?
Endonucleases are classified in to ________ main classes.
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