Multiple Choice Questions (MCQs)
Two pair of enzymes are used in ________ PCR.
Agarose extracted from sea weeds finds use in ________.
________ enzymes have symmetrical recognition sequences but are of little value.
________ is used as a template to produce DNA in Reverse Transcripatase PCR.
Parental genetic diagnosis is not important for identification of ________.
Real Time PCR is used to measure the quantity of a ________.
________ enzymes have symmetrical recognition sequencesbut are of little value.
If the primer sequence is TCG, what section of DNA will it anneal?
Primers used for the process of polymerase chain reaction are ________.
Genomic fingerprinting is a ________ medicine technique used to identify individuals by characteristics of their DNA.
The process of reverse transcription reverse transcribes ________.
Which one of the following is the source of pfu Polymerase?
The extension rate of Taq Polymerase enzyme is ________.
Binding of primers to the single stranded DNA is called ________.
________ is used for the screening of bacterial of yeast clones for correct ligation or plasmid products.
Why are vent ploymerase and Pfu more efficient than the Taq polymerase?
Allele-specific PCR used for identifying of ________.
Melting of DNA template results in yielding ________ of DNA.
What would the generally expected effect on the PCR reaction be of adjustments that increase the temperature of the annealing phase and the length of the elongation phase?
________ PCR uses primers whose 3' ends encompass the SNP.
At what temperature does annealing of DNA and primer take place?
Which of the following statements are true regarding PCR?
Non genomic material such as RNA can be detected by ________.
In ________ is a polymerase chain reaction that actually takes place inside the cell on a slide
A technique for making millions of copies of a specific region of DNA.
What is the function of a primer?
________ refers to the study of ancient human, animal and plant remains.
For making cDNA ________ is used as a template.
Enzyme that creates phosphodiester bond between 3' end of one DNA segment and the 5' another is known as ________.
Methylation-specific PCR is used to identify patterns of DNA methylation at ________.
What happens during the Anneal step of PCR?
A recognition sequence usually ranges from ________ bases.
Two sets of primers instead of one pair are uses in ________.
AFLP is a highly sensitive PCR-based method for detecting ________ in DNA.
________ PCR uses the template for the reverse primers is a restriction fragment that has been self-ligated.
Degenerate Primers are used for the amplication of ________.
Denaturation is the process of ________.
The limitations of PCR are ________.
At what temperature does denaturation of DNA double helix takes place?
In ________ PCR there is very low probability of non-specific amplification of DNA.
What happens in the Denature step of PCR?
Molecular Detection methods can facilitate in detection of ________ in plants, animals and environment.
________ PCR is used for screening of bacterial or yeast clones for correct ligation or plasmid products.
Inverse PCR is used for the identification and amplification of ________.
Suicide PCR is used in ________.
Inverse PCR supports the amplification and identification of ________.
To amplify one strand of the original DNA more than the other, a special type of PCR is used which is known as ________.
________ tools have also allowed to perform prenatal genetic diagnosis.
A heat stable enzyme called Taq Polymerase is used in Polymerase Chain Reaction (PCR) which is obtained from a bacterium ________.
Sequences of nucleotides which have a twofold axis of rotational symmetry is known as ________.
The instrument used to run the PCR is known as ________.
Endonucleases are classified in to ________ main classes.
Mini Primer PCR uses a thermostable polymerase (S-Tbr) that can extend from short primers as short as ________ nucleotides.
________ tools have also allowed to perform penatal genetic diagnosis.
Assembly PCR is used to assemble two or more places of DNA into ________.
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