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Molecular Biology (BIO302)

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Course Contents

Introduction to Molecular Biology: Reviewing fundamental concepts about molecular biology, Basic concepts about DNA: Chemical composition & structure of DNA, Basic concepts about RNA: Chemical composition & structure of RNA, Basic concepts about Proteins: Chemical composition & structure of Proteins, Nature of Genetic Material: Experiments proving that DNA is the genetic material (Griffith’s experiments etc.), Organization of genetic material in Viruses: Nucleic acids in phages, genomes of animal & plant viruses, Organization of genetic material in Prokaryotes: The structure and organization of bacterial chromatin and bacterial chromosomes, Organization of genetic material in Eukaryotes: The structure and organization of eukaryotic chromosomes, nucleosomes, histones, Central dogma of life: Description of the Flow of genetic information, replication, transcription, reverse transcription and translation, DNA Replication: (Chemistry of DNA synthesis, DNA Polymerase, Replication fork, Initiation of Replication, Binding & unwinding, Finishing replication), DNA mutation: (Nature of mutations, Spontaneous mutations by hydrolysis & deamination, Mutations by alkylation, Mutations by UV radiations, Mutations by Gamma & X-rays, Mutations by base analogs & intercalating agents), DNA repair:(Direct reversal of DNA damage, Base Excision Repair, Nucleotide Excision Repair, Recombination Repairs, Direct joining of broken ends), Transcription (RNA Polymerases & Transcription cycle, Transcription cycle in Bacteria, Transcription cycle in Eukaryotes), RNA Splicing: (Chemistry of RNA splicing, Spliceosome Machinery, Splicing Pathways, Exon Shuffling), RNA Editing: Altering the sequence of mRNA, Translation: (Initiation of Translation, Elongation of Translation, Termination of Translation), Post Translational modifications: Nascent polypeptide processing & folding, Regulatory Elements: (Regulation by RNAs in bacteria, Promotors, Enhancers & Silencers), Regulation of gene expression : (mRNA, Lactose Operon, Catabolite Repression, Galactose Operon, Tryptophan Operon), Control of Gene Expression in Prokaryotes: (Gene expression is controlled by regulatory proteins), Introduction to Plasmids: (Introduction to different types of vectors used in molecular biology), Introduction to Vectors: Introduction to different types of vectors used in molecular biology.

Practical Contents:
Lab safety protocols Lab equipment
Preparation of stock solutions.
Preparation of working solutions.
Isolation of nucleic acids.
Quantification of nucleic acids
Restriction digestion of DNA
Preparation of restriction maps.
Gel electrophoresis
Polymerase chain reaction (PCR).
Detection of mutations
Restriction fragment length polymorphism.
Preparation of chemically competent cells.
Transformation of bacteria with plasmid DNA.
Comparing plasmids of different molecular weights
Molecular weights marker

Course Codes

ACC311 ACC501 BIF401 BIF402 BIF501 BIF601 BIF602 BIF604 BIF731 BIF732 BIF733 BIO101 BIO102 BIO201 BIO202 BIO203 BIO204 BIO301 BIO302 BIO303 BIO502 BIO731 BIO732 BIO733 BIO734 BIT701 BIT703 BIT710 BIT715 BNK601 BNK603 BNK604 BNK610 BNK611 BNK612 BNK613 BNK701 BNK703 BNK704 BNK725 BT101 BT102 BT301 BT302 BT404 BT501 BT503 BT505 BT601 BT603 BT605 BT731 BT732 BT733 BT734 BT735 CS001 CS101 CS201 CS202 CS205 CS206 CS301 CS302 CS304 CS310 CS311 CS312 CS314 CS315 CS401 CS402 CS403 CS405 CS407 CS408 CS409 CS410 CS411 CS431 CS432 CS435 CS501 CS502 CS504 CS506 CS507 CS508 CS510 CS601 CS602 CS603 CS604 CS605 CS606 CS607 CS608 CS609 CS610 CS611 CS614 CS615 CS620 CS701 CS702 CS703 CS704 CS706 CS707 CS708 CS709 CS710 CS711 CS712 CS713 CS716 CS718 CS721 CS723 CS724 CS725 CS726 ECE101 ECE202 ECE301 ECO302 ECO303 ECO401 ECO402 ECO403 ECO404 ECO406 ECO501 ECO601 ECO602 ECO603 ECO606 ECO615 ECO704 EDU101 EDU201 EDU301 EDU303 EDU304 EDU305 EDU401 EDU402 EDU403 EDU404 EDU405 EDU406 EDU410 EDU411 EDU430 EDU431 EDU501 EDU505 EDU510 EDU512 EDU515 EDU516 EDU601 EDU602 EDU603 EDU604 EDU654 EDU702 EDU705 ENG001 ENG101 ENG201 ENG301 ENG401 ENG503 ENG504 ENG505 ENG506 ENG507 ENG508 ENG509 ENG510 ENG511 ENG512 ENG513 ENG515 ENG516 ENG518 ENG519 ETH201 ETH202 FIN611 FIN621 FIN622 FIN623 FIN624 FIN625 FIN630 FIN704 FIN711 FIN722 FIN723 FIN725 FIN730 GEN731 GEN732 GSC101 GSC201 HRM611 HRM613 HRM617 HRM623 HRM624 HRM625 HRM626 HRM627 HRM628 HRM713 HRM724 HRM727 ISL201 IT430 MCD401 MCD402 MCD403 MCD501 MCD502 MCD504 MCM101 MCM301 MCM304 MCM310 MCM311 MCM401 MCM404 MCM411 MCM431 MCM501 MCM511 MCM514 MCM515 MCM516 MCM517 MCM520 MCM531 MCM532 MCM601 MCM604 MCM610 MGMT510 MGMT611 MGMT614 MGMT615 MGMT617 MGMT622 MGMT623 MGMT625 MGMT627 MGMT628 MGMT629 MGMT630 MGMT631 MGMT715 MGMT723 MGMT725 MGMT727 MGMT728 MGMT729 MGMT730 MGMT731 MGT101 MGT111 MGT201 MGT211 MGT301 MGT401 MGT402 MGT404 MGT411 MGT501 MGT502 MGT503 MGT504 MGT510 MGT513 MGT520 MGT522 MGT601 MGT602 MGT603 MGT604 MGT610 MGT611 MGT612 MGT613 MGT621 MGT703 MGT704 MGT705 MGT711 MGT713 MGT722 MKT501 MKT529 MKT530 MKT610 MKT611 MKT621 MKT624 MKT625 MKT627 MKT630 MKT703 MKT711 MKT721 MKT724 MKT725 MKT730 MKT731 MTH001 MTH100 MTH101 MTH201 MTH202 MTH301 MTH302 MTH303 MTH401 MTH403 MTH501 MTH601 MTH603 MTH631 MTH632 MTH633 MTH634 MTH641 MTH701 MTH706 MTH7123 MTH721 PAD603 PAK301 PAK302 PHY101 PHY301 PSC201 PSC401 PSY101 PSY401 PSY402 PSY403 PSY404 PSY405 PSY406 PSY407 PSY408 PSY409 PSY502 PSY504 PSY505 PSY510 PSY511 PSY512 PSY513 PSY514 PSY515 PSY610 PSY631 PSY632 SOC101 SOC301 SOC302 SOC401 SOC402 SOC403 SOC601 SOC603 SOC615 SPT730 STA100 STA301 STA304 STA404 STA406 STA408 STA621 STA630 STA631 STA632 STA642 STA643 STA644 STA730 STAT730 URD101 ZOO301 ZOO502 ZOO503 ZOO504 ZOO505 ZOO731